Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging

Title
Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging
Author
임동우
Keywords
Surface-enhanced Raman scattering; SERS nano tag; Multiplex detection; Breast cancer; Cell phenotype; RECEPTOR MONOCLONAL-ANTIBODY; LUNG-CANCER; CIXUTUMUMAB IMC-A12; SPECTROSCOPIC TAGS; GOLD NANOPARTICLES; SINGLE-MOLECULE; ERBB RECEPTORS; LIVE CELLS; NANOPROBES; IMMUNOASSAY
Issue Date
2014-01
Publisher
ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
Citation
BIOSENSORS & BIOELECTRONICS; JAN 15 2014, Vol.51 ,p238-p243, 6p.
Abstract
We report a surface-enhanced Raman scattering (SERS)-based cellular imaging technique to detect and quantify breast cancer phenotypic markers expressed on cell surfaces. This technique involves the synthesis of SERS nano tags consisting of silica-encapsulated hollow gold nanospheres (SEHGNs) conjugated with specific antibodies. Hollow gold nanospheres (HGNs) enhance SERS signal intensity of individual particles by localizing surface electromagnetic fields through pinholes in the hollow particle structures. This capacity to enhance imaging at the level of single molecules permits the use of HGNs to detect specific biological markers expressed in living cancer cells. In addition, silica encapsulation greatly enhances the stability of nanoparticles. Here we applied a SERS-based imaging technique using SEHGNs in the multiplex imaging of three breast cancer cell phenotypes. Expression of epidermal growth factor (EGF), ErbB2, and insulin-like growth factor-1 (IGF-1) receptors were assessed in the MDA-MB-468, KPL4 and SK-BR-3 human breast cancer cell lines. SERS imaging technology described here can be used to test the phenotype of a cancer cell and quantify proteins expressed on the cell surface simultaneously. Based on results, this technique may enable an earlier diagnosis of breast cancer than is currently possible and offer guidance in treatment. (C) 2013 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0956566313005356http://hdl.handle.net/20.500.11754/47500
ISSN
0956-5663
DOI
10.1016/j.bios.2013.07.063
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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