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dc.contributor.author안주홍-
dc.date.accessioned2018-03-15T07:38:00Z-
dc.date.available2018-03-15T07:38:00Z-
dc.date.issued2014-06-
dc.identifier.citationThe international journal of biochemistry & cell biology, 2014, 53, P.399-408en_US
dc.identifier.issn1357-2725-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1357272514002039?via%3Dihub-
dc.description.abstractExcessive accumulation of unfolded proteins in the endoplasmic reticulum (ER) lumen causes ER stress, which induces a set of genes, including those encoding ER-resident chaperones, to relieve the detrimental effects and recover homeostasis. Calreticulin is a chaperone that facilitates protein folding in the ER lumen, and its gene expression is induced by ER stress in Caenorhabditis elegans. Sumoylation conjugates small ubiquitin-like modifier (SUMO) proteins with target proteins to regulate a variety of biological processes, such as protein stability, nuclear transport, DNA binding, and gene expression. In this study, we showed that C elegans X-box-binding protein 1 (Ce-XBP-1), an ER stress response transcription factor, interacts with the SUMO-conjugating enzyme UBC-9 and a SUMOylation target. Our results indicated that abolishing sumoylation enhanced calreticulin expression in an XBP-1-dependent manner, and the resulting increase in calreticulin counteracted ER stress. Furthermore, sumoylation was repressed in C. elegans undergoing ER stress. Finally, RNAi against ubc-9 mainly affected the expression of genes associated with ER functions, such as lipid and organic acid metabolism. Our results suggest that sumoylation plays a regulatory role in ER function by controlling the expression of genes required for ER homeostasis in C elegans. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipSome strains are provided by the Caenorhabditis Genetic Center, which is funded by NIH office of Research Infrastructure Programs (P40 OD010440) and National Bioresource Project (NBP-Japan). This work was supported by the 2014 GIST Systems Biology Infrastructure Establishment Grant, the Women Scientist program (no. 2013R1A1A3A04006010) in the Basic Science Research Program and the Bio & Medical Technology Development Program (NRF-2013M3A9A7046297) of the National Research Foundation of Korea (NRF) funded by Korean Ministry of Science, ICT & Future Planning.en_US
dc.language.isoenen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.subjectSumoylationen_US
dc.subjectXBP-1en_US
dc.subjectUBC-9en_US
dc.subjectCalreticulinen_US
dc.subjectER stressen_US
dc.subjectUNFOLDED PROTEIN RESPONSEen_US
dc.subjectENDOPLASMIC-RETICULUMen_US
dc.subjectOXIDATIVE STRESSen_US
dc.subjectMESSENGER-RNAen_US
dc.subjectSUMOen_US
dc.subjectCALCIUMen_US
dc.subjectDEATHen_US
dc.subjectSMT3en_US
dc.subjectXBP1en_US
dc.titleSumoylation regulates ER stress response by modulating calreticulin gene expression in XBP-1-dependent mode in Caenorhabditis elegansen_US
dc.typeArticleen_US
dc.relation.volume53-
dc.identifier.doi10.1016/j.biocel.2014.06.005-
dc.relation.page399-408-
dc.relation.journalINTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY-
dc.contributor.googleauthorLim, Yunki-
dc.contributor.googleauthorLee, Dukgyu-
dc.contributor.googleauthorKalichamy, Karunambigai-
dc.contributor.googleauthorHong, Seong-Eui-
dc.contributor.googleauthorMichalak, Marek-
dc.contributor.googleauthorAhnn, Joohong-
dc.contributor.googleauthorKim, Do Han-
dc.contributor.googleauthorLee, Sun-Kyung-
dc.relation.code2014031343-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidjoohong-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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