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dc.contributor.author김연제-
dc.date.accessioned2018-03-15T07:20:54Z-
dc.date.available2018-03-15T07:20:54Z-
dc.date.issued2014-06-
dc.identifier.citationBIOTECHNOLOGY LETTERS, 권: 36, 호: 10, 페이지: 2069-2077en_US
dc.identifier.issn0141-5492-
dc.identifier.issn1573-6776-
dc.identifier.urihttp://link.springer.com/article/10.1007%2Fs10529-014-1578-2-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/47335-
dc.description.abstractPinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 mu g +/- A 7 alpha-pinene g(-1) cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.en_US
dc.description.sponsorshipThe authors thank Prof. Andreas Burkovski for providing the LN Delta GDH mutant and Ms. Jae Hee Jung for technical support. This work was supported by the National Research Foundation of Korea grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute cooperation program) and Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/Korea Institute of Science and Technology (KIST) (Project No. 2E24832).en_US
dc.language.isoenen_US
dc.publisherSPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSen_US
dc.subjectCorynebacterium Glutamicumen_US
dc.subjectMetabolic Engineeringen_US
dc.subjectMonoterpeneen_US
dc.subjectPineneen_US
dc.subjectToxicityen_US
dc.titleBiosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicumen_US
dc.typeArticleen_US
dc.relation.volume36-
dc.identifier.doi10.1007/s10529-014-1578-2-
dc.relation.page2069-2077-
dc.relation.journalBIOTECHNOLOGY LETTERS-
dc.contributor.googleauthorKang, Min-Kyoung-
dc.contributor.googleauthorEom, Jin-Hee-
dc.contributor.googleauthorKim, Yun-je-
dc.contributor.googleauthorUm, Young-soon-
dc.contributor.googleauthorWoo, Han-Min-
dc.relation.code2014026241-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentRESEARCH INSTITUTE OF INDUSTRIAL SCIENCE-
dc.identifier.pidyjkim4070-
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RESEARCH INSTITUTE[S](부설연구소) > RESEARCH INSTITUTE OF INDUSTRIAL SCIENCE(산업과학연구소) > Articles
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