Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum

Title
Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum
Authors
김연제
Keywords
Corynebacterium Glutamicum; Metabolic Engineering; Monoterpene; Pinene; Toxicity
Issue Date
2014-06
Publisher
SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Citation
BIOTECHNOLOGY LETTERS, 권: 36, 호: 10, 페이지: 2069-2077
Abstract
Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 mu g +/- A 7 alpha-pinene g(-1) cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.
URI
http://link.springer.com/article/10.1007%2Fs10529-014-1578-2http://hdl.handle.net/20.500.11754/47335
ISSN
0141-5492; 1573-6776
DOI
http://dx.doi.org/10.1007/s10529-014-1578-2
Appears in Collections:
RESEARCH INSTITUTE[S](부설연구소) > RESEARCH INSTITUTE OF INDUSTRIAL SCIENCE(산업과학연구소) > Articles
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