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dc.contributor.author박범수-
dc.date.accessioned2018-03-15T06:59:15Z-
dc.date.available2018-03-15T06:59:15Z-
dc.date.issued2014-07-
dc.identifier.citationHarmful Algae, 2014, 37, P.133-141en_US
dc.identifier.issn1568-9883-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1568988314000754?via%3Dihub-
dc.description.abstractWhile the toxic dinoflagellate Cochlodinium polykrikoides is known to form blooms that are maintained for extended periods, the genetic differentiation of these blooms are currently unknown. To assess this, we developed a real-time PCR assay to quantify C polykrikoides at the intra-specific level, and applied this assay to field samples collected in Korean coastal waters from summer through fall. Assays were successfully developed to target the large-subunit ribosomal RNA region of the three major ribotypes of C polykrikoides: Philippines, East Asian, and American/Malaysian. Significant linear relationships (r(2) >= 0.995) were established between C-t and the log of the copy number for each ribotype qPCR assay. Using these assays, C. polykrikoides blooms in Korean coastal waters were found to be comprised of Philippines and East Asian ribotypes but not the American/Malaysian ribotype. The Philippines ribotype was found to be highly abundant during summer bloom initiation and peak, whereas the East Asian ribotype became the dominant ribotype in the fall. As such, this newly developed qPCR assay can be used to quantify the cryptic ecological succession of sub-populations of C polykrikoides during blooms that light microscopy and previously developed qPCR assays cannot resolve.en_US
dc.description.sponsorshipWe thank Dr. Ying Zhong Tang for providing the Cochlodinium polykrikoides strain CP01, Myo-Kyung Kim, Zhun Li for assistance with field work, and Dr. Jang-Seu Ki and Dr. Sunju Kim for providing kind comments. This research was supported by a grant from Marine Biotechnology Program Funded by Ministry of Oceans and Fisheries, Korea.[SS]en_US
dc.language.isoenen_US
dc.publisherElsevier Science B.Ven_US
dc.subjectCochlodinium polykrikoidesen_US
dc.subjectRibotypeen_US
dc.subjectQuantitative real-time PCRen_US
dc.subjectField applicationen_US
dc.titleResolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assaysen_US
dc.typeArticleen_US
dc.relation.volume37-
dc.identifier.doi10.1016/j.hal.2014.04.019-
dc.relation.page133-141-
dc.relation.journalHARMFUL ALGAE-
dc.contributor.googleauthorPark, Bum Soo-
dc.contributor.googleauthorWang, Pengbin-
dc.contributor.googleauthorKim, Jin Ho-
dc.contributor.googleauthorKim, Joo-Hwan-
dc.contributor.googleauthorGobler, Christopher J-
dc.contributor.googleauthorHan, Myung-Soo-
dc.relation.code2014030251-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentTHE RESEARCH INSTITUTE FOR NATURAL SCIENCES-
dc.identifier.pidparkbs-
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