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Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays

Title
Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays
Author
박범수
Keywords
Cochlodinium polykrikoides; Ribotype; Quantitative real-time PCR; Field application
Issue Date
2014-07
Publisher
Elsevier Science B.V
Citation
Harmful Algae, 2014, 37, P.133-141
Abstract
While the toxic dinoflagellate Cochlodinium polykrikoides is known to form blooms that are maintained for extended periods, the genetic differentiation of these blooms are currently unknown. To assess this, we developed a real-time PCR assay to quantify C polykrikoides at the intra-specific level, and applied this assay to field samples collected in Korean coastal waters from summer through fall. Assays were successfully developed to target the large-subunit ribosomal RNA region of the three major ribotypes of C polykrikoides: Philippines, East Asian, and American/Malaysian. Significant linear relationships (r(2) >= 0.995) were established between C-t and the log of the copy number for each ribotype qPCR assay. Using these assays, C. polykrikoides blooms in Korean coastal waters were found to be comprised of Philippines and East Asian ribotypes but not the American/Malaysian ribotype. The Philippines ribotype was found to be highly abundant during summer bloom initiation and peak, whereas the East Asian ribotype became the dominant ribotype in the fall. As such, this newly developed qPCR assay can be used to quantify the cryptic ecological succession of sub-populations of C polykrikoides during blooms that light microscopy and previously developed qPCR assays cannot resolve.
URI
https://www.sciencedirect.com/science/article/pii/S1568988314000754?via%3Dihub
ISSN
1568-9883
DOI
10.1016/j.hal.2014.04.019
Appears in Collections:
RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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