Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
- Title
- Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
- Author
- 주재범
- Keywords
- anisotropy; biohybrid microparticles; electrohydrodynamic co-jetting; multiplexed biosensing; sandwich immunocomplex
- Issue Date
- 2014-01
- Publisher
- WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
- Citation
- Macromolecular Rapid Communications, Volume 35, Issue 1, Pages 56?65
- Abstract
- Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system.
- URI
- http://onlinelibrary.wiley.com/doi/10.1002/marc.201300652/fullhttp://hdl.handle.net/20.500.11754/47230
- ISSN
- 1521-3927
- DOI
- 10.1002/marc.201300652
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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