Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2018-03-15T06:30:24Z | - |
dc.date.available | 2018-03-15T06:30:24Z | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | Macromolecular Rapid Communications, Volume 35, Issue 1, Pages 56?65 | en_US |
dc.identifier.issn | 1521-3927 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1002/marc.201300652/full | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/47230 | - |
dc.description.abstract | Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system. | en_US |
dc.description.sponsorship | Funded by Ministry of Education, Science, and Technology, Korea. Grant Numbers: 2010?0013284, 2012R1A1A2043792, 2012?0009838, R11?2008?044?01002?0, Ministry of Science, ICT and Future Planning, Korea. Grant Number: 2013R1A2A2A04016796, Agency for Defense Development | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | en_US |
dc.subject | anisotropy | en_US |
dc.subject | biohybrid microparticles | en_US |
dc.subject | electrohydrodynamic co-jetting | en_US |
dc.subject | multiplexed biosensing | en_US |
dc.subject | sandwich immunocomplex | en_US |
dc.title | Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing | en_US |
dc.type | Article | en_US |
dc.relation.volume | 35 | - |
dc.identifier.doi | 10.1002/marc.201300652 | - |
dc.relation.page | 56-65 | - |
dc.relation.journal | MACROMOLECULAR RAPID COMMUNICATIONS | - |
dc.contributor.googleauthor | Jung, Woo | - |
dc.contributor.googleauthor | Jalani, Ghulam | - |
dc.contributor.googleauthor | Ko, Juhui | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.contributor.googleauthor | Lim, Dong Woo | - |
dc.relation.code | 2014035552 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
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