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dc.contributor.author주재범-
dc.date.accessioned2018-03-15T06:30:24Z-
dc.date.available2018-03-15T06:30:24Z-
dc.date.issued2014-01-
dc.identifier.citationMacromolecular Rapid Communications, Volume 35, Issue 1, Pages 56?65en_US
dc.identifier.issn1521-3927-
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1002/marc.201300652/full-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/47230-
dc.description.abstractAnisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system.en_US
dc.description.sponsorshipFunded by Ministry of Education, Science, and Technology, Korea. Grant Numbers: 2010?0013284, 2012R1A1A2043792, 2012?0009838, R11?2008?044?01002?0, Ministry of Science, ICT and Future Planning, Korea. Grant Number: 2013R1A2A2A04016796, Agency for Defense Developmenten_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USAen_US
dc.subjectanisotropyen_US
dc.subjectbiohybrid microparticlesen_US
dc.subjectelectrohydrodynamic co-jettingen_US
dc.subjectmultiplexed biosensingen_US
dc.subjectsandwich immunocomplexen_US
dc.titleSynthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensingen_US
dc.typeArticleen_US
dc.relation.volume35-
dc.identifier.doi10.1002/marc.201300652-
dc.relation.page56-65-
dc.relation.journalMACROMOLECULAR RAPID COMMUNICATIONS-
dc.contributor.googleauthorJung, Woo-
dc.contributor.googleauthorJalani, Ghulam-
dc.contributor.googleauthorKo, Juhui-
dc.contributor.googleauthorChoo, Jaebum-
dc.contributor.googleauthorLim, Dong Woo-
dc.relation.code2014035552-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjbchoo-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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