Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing

Title
Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
Author
주재범
Keywords
anisotropy; biohybrid microparticles; electrohydrodynamic co-jetting; multiplexed biosensing; sandwich immunocomplex
Issue Date
2014-01
Publisher
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Citation
Macromolecular Rapid Communications, Volume 35, Issue 1, Pages 56?65
Abstract
Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system.
URI
http://onlinelibrary.wiley.com/doi/10.1002/marc.201300652/fullhttp://hdl.handle.net/20.500.11754/47230
ISSN
1521-3927
DOI
10.1002/marc.201300652
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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