Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds

Title
Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds
Author
상병인
Keywords
Lignin; Hydrolysate; Transcriptome; Biomarker; Ferulic acid
Issue Date
2012-11
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Bioresource Technology, 2012, 114, P.450-456
Abstract
Aberrations in the growth and transcriptome of Escherichia coli str. BL21(DE3) were determined when exposed to varying concentrations of ferulic acid (0.25-1 g/L), an aromatic carboxylic acid identified within lignin-cellulose hydrolysate samples. The expression of several individual genes (aaeA, aaeB, inaA and marA) was significantly induced, i.e., more than 4-fold, and thus these genes and the heat shock response gene htpG were selected as biomarkers to monitor E. coli's responses to five additional hydrolysate-related compounds, including vanillic acid, coumaric acid, 4-hydroxybenzoic acid, ferulaldehyde and furfural. While all of the biomarkers showed dose-dependent responses to most of the compounds, expression of aaeA and aaeB showed the greatest induction (5-30-fold) for all compounds tested except furfural. Lastly, the marA, inaA and htpG genes all showed higher expression levels when the culture was exposed to spruce hydrolysate samples, demonstrating the potential use of these genes as biomarkers. (C) 2012 Elsevier Ltd. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0960852412003367?via%3Dihubhttp://hdl.handle.net/20.500.11754/47053
ISSN
0960-8524
DOI
10.1016/j.biortech.2012.02.085
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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