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An Image Cytometric MTT Assay as an Alternative Assessment Method of Nanoparticle Cytotoxicity

Title
An Image Cytometric MTT Assay as an Alternative Assessment Method of Nanoparticle Cytotoxicity
Author
윤태현
Keywords
Alternative assay method; Nanoparticles cytotoxicity; Image cytometry; MTT assay; TiO2 nanoparticles; CARBON NANOTUBES; TIO2 NANOPARTICLES; TOXICITY; DISPERSION; CELLS
Issue Date
2014-07
Publisher
Korean Chemical SOC
Citation
Bulletin of the Korean Chemical Society, 2014, 35(7), P.1933-1938
Abstract
Despite increasing importance of in vitro cell-based assays for the assessment of nanoparticles (NPs) cytotoxicity, their suitability for the assessment of NPs toxicity is still in doubt. Here, limitations of widely used cell viability assay protocol (i.e., MTT asssay) for the cytotoxicity assessment of P25 TiO2 NPs were carefully examined and an alternative toxicity assessment method to overcome these limitations was proposed, where the artifacts caused by extracellularly formed formazan and light scattered by agglomerated NPs were minimized by measuring only the intracellular formazan via image cytometric methods.
URI
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=JAKO201420947475565&SITE=CLICKhttp://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=JCGMCS_2014_v35n7_1933
ISSN
0253-2964; 1225-0058; 1229-5949
DOI
10.5012/bkcs.2014.35.7.1933
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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