Comparison of Sensitivity of Enzyme Immunoassays for Toxin A and B in different C. difficile PCR Ribotypes

Title
Comparison of Sensitivity of Enzyme Immunoassays for Toxin A and B in different C. difficile PCR Ribotypes
Author
이양순
Keywords
MOLECULAR EPIDEMIOLOGY; SOUTH-KOREA; INFECTIONS; RESISTANCE; TESTS; Bacterial Toxins; metabolism; Clostridium difficile; isolation & purification; Enterotoxins; Humans; Immunoenzyme Techniques; methods; Polymerase Chain Reaction; Ribotyping; Sensitivity and Specificity
Issue Date
2014-01
Publisher
ASSOC CLINICAL SCIENTISTS, PO BOX 1287, MIDDLEBURY, VT 05753 USA
Citation
Annals of Clinical & Laboratory Science. Dec 15, 2014, Vol. 44, No. 1, p.38-41
Abstract
Enzyme immunoassays (EIAs) for toxins A and B are the most common assays for the diagnosis of Clostridium difficile infection due to their rapidity and ease of use. However, the sensitivity of different kits varies greatly. The predominant PCR ribotypes of C. difficile vary according to the region or country studied, and it was recently reported that the sensitivity of EIAs can be affected by the strain type. The aim of this study was to assess the sensitivity of EIAs in different PCR ribotypes of C. difficile during a period of five years in a Korean hospital. A total of 969 toxigenic C. difficile isolates were recovered from patients with diarrhea in a hospital from 2006 to 2009 (inclusive), and 2011. Overall sensitivities of Tox A/B Quik Chek (TechLab, Blacksburg, VA) and VIDAS C. difficile A & B (bioMerieux, Marcy l'Etoile, France) were 36.4% and 46.3%, respectively. The sensitivities of TOX A/B Quick Chek and VIDAS Clostridium difficile A & B for the five most common ribotypes were as follows: 56.6% and 71.7% for ribotype AB17 (ribotype 018); 48.6% and 54.3% for ribotype aB (ribotype 017); 25.3% and 36.3% for ribotype AB2 (ribotype 014); 13.0% and 24.2% for ribotype AB3; 66.7% and 0% for ribotype AB1 (ribotype 001), respectively. The sensitivity for the predominant ribotype, AB17, was significantly different from those for aB, AB2, AB1, and AB3 using VIDAS Clostridium difficile A & B (p<0.05). These data suggest that the sensitivity of EIA may be affected by the distribution of ribotypes. ⓒ 2014 by the Association of Clinical Scientists, Inc.
URI
http://www.annclinlabsci.org/content/44/1/38.fullhttp://hdl.handle.net/20.500.11754/46657
ISSN
0091-7370; 1550-8080
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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