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dc.contributor.author박준성-
dc.date.accessioned2018-03-14T03:06:42Z-
dc.date.available2018-03-14T03:06:42Z-
dc.date.issued2014-07-
dc.identifier.citationKIDNEY & BLOOD PRESSURE RESEARCH, 2014, 39(1), P.86-96en_US
dc.identifier.issn1420-4096-
dc.identifier.urihttps://www.karger.com/Article/FullText/355782-
dc.description.abstractBackground/Aims: Although high salt intake is thought to accelerate renal progression in proteinuric kidney disease, it is not known whether strict dietary salt restriction could delay renal inflammation and interstitial fibrosis. Here, we sought to answer this question in a rat model of adriamycin-induced nephrotic syndrome. Methods: Adriamycin was administered via the femoral vein in a single bolus (7.5 mg/kg), and the rats were put on a sodium-deficient rodent diet. Rats with intact kidneys were studied for 5 weeks (experiment 1), and uninephrectomized rats were studied for 6 weeks (experiment 2). Results: In experiment 1, restricting salt intake improved renal tubulointerstitial histopathology in adriamycin-treated rats. Immunohistochemical and immunoblot results additionally showed that restricting dietary salt lowered adriamycin-induced expression of osteopontin, collagen III, and fibronectin. In experiment 2, salt restriction improved adriamycin-induced azotemia, although it did not affect proteinuria or blood pressure. Dietary salt restriction also reduced adriamycin-induced infiltration of ED1-positive cells and the upregulated expression of osteopontin and alpha-SMA. Masson's trichrome and Sirius red staining revealed that salt restriction slowed adriamycin-induced progression of renal interstitial fibrosis. Finally, qPCR revealed that adriamycin-induced expression of TNF-alpha, I.B-alpha, gp91(phox), p47(phox), and p67(phox) mRNA was blocked by salt restriction. Conclusion: Our findings demonstrate that strict dietary salt restriction delays the progress of renal inflammation and fibrosis in proteinuric kidney disease, most likely via relieving the reactive oxygen species-mediated NF-kappa B activation. Copyright (C) 2014 S. Karger AG, Baselen_US
dc.description.sponsorshipBackground/Aims: Although high salt intake is thought to accelerate renal progression in proteinuric kidney disease, it is not known whether strict dietary salt restriction could delay renal inflammation and interstitial fibrosis. Here, we sought to answer this question in a rat model of adriamycin-induced nephrotic syndrome. Methods: Adriamycin was administered via the femoral vein in a single bolus (7.5 mg/kg), and the rats were put on a sodium-deficient rodent diet. Rats with intact kidneys were studied for 5 weeks (experiment 1), and uninephrectomized rats were studied for 6 weeks (experiment 2). Results: In experiment 1, restricting salt intake improved renal tubulointerstitial histopathology in adriamycin-treated rats. Immunohistochemical and immunoblot results additionally showed that restricting dietary salt lowered adriamycin-induced expression of osteopontin, collagen III, and fibronectin. In experiment 2, salt restriction improved adriamycin-induced azotemia, although it did not affect proteinuria or blood pressure. Dietary salt restriction also reduced adriamycin-induced infiltration of ED1-positive cells and the upregulated expression of osteopontin and alpha-SMA. Masson's trichrome and Sirius red staining revealed that salt restriction slowed adriamycin-induced progression of renal interstitial fibrosis. Finally, qPCR revealed that adriamycin-induced expression of TNF-alpha, I.B-alpha, gp91(phox), p47(phox), and p67(phox) mRNA was blocked by salt restriction. Conclusion: Our findings demonstrate that strict dietary salt restriction delays the progress of renal inflammation and fibrosis in proteinuric kidney disease, most likely via relieving the reactive oxygen species-mediated NF-kappa B activation.en_US
dc.language.isoko_KRen_US
dc.publisherKARGERen_US
dc.subjectFibrosisen_US
dc.subjectDietary NaClen_US
dc.subjectDoxorubucinen_US
dc.subjectNF-kappa Ben_US
dc.subjectNADPH oxidaseen_US
dc.titleEffects of Dietary Salt Restriction on Renal Progression and Interstitial Fibrosis in Adriamycin Nephrosisen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume39-
dc.identifier.doi10.1159/000355782-
dc.relation.page86-96-
dc.relation.journalKIDNEY & BLOOD PRESSURE RESEARCH-
dc.contributor.googleauthorPark, Joon-Sung-
dc.contributor.googleauthorKim, Sua-
dc.contributor.googleauthorJo, Chor Ho-
dc.contributor.googleauthorOh, Il Hwan-
dc.contributor.googleauthorKim, Gheun-Ho-
dc.relation.code2014035175-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidsjpjoon-


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