Lobarstin Enhances Chemosensitivity in Human Glioblastoma T98G Cells

Title
Lobarstin Enhances Chemosensitivity in Human Glioblastoma T98G Cells
Author
조성신
Keywords
Lobarstin; glioblastoma; temozolomide; chemosensitivity; DNA repair
Issue Date
2013-11
Publisher
INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH
Citation
Anticancer Research, Vol.33, No.12 [2013], p5445-5452
Abstract
Background/Aim: Lobarstin is a metabolite occurring from the Antarctic lichen Stereocaulon alpnum. Human glioblastoma is highly resistant to chemotherapy with temozolomide. Lobarstin was examined for its effect on glioblastoma. Materials and Methods: Temozolomide-resistant T98G cells were subjected to toxicity test with temozolomide and/or lobarstin. DNA damage and recovery was assessed by the alkaline comet assay and expression of DNA repair genes was examined by RT-PCR and western blot analysis. Results: Lobarstin alone at 40 mu M was toxic against T98G, but had no effect in primary human fibroblasts. Co-treatment of lobarstin with temozolomide yielded enhanced toxicity. Temozolomide-alone or with lobarstin co-treatment gave similar extent of DNA damage. However, the recovery was reduced in co-treated cells. Expression of DNA repair genes, O-6-methylguanine-DNA methyltransferase, poly(ADPribose) polymerase I and ligase 3 were reduced in lobarstin-treated cells. Conclusion: Enhanced sensitivity to temozolomide by lobarstin co-treatment may be attributed to reduced DNA repair.
URI
http://ar.iiarjournals.org/content/33/12/5445.shorthttp://hdl.handle.net/20.500.11754/46320
ISSN
0250-7005
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RESEARCH INSTITUTE[S](부설연구소) > ETC
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