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A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction

Title
A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction
Author
Jong Wook Hong
Keywords
Prostate specific antigen (PSA); Au nanoparticle; Nanobiosensor; Enzymatic cleavage reaction; Protein detection
Issue Date
2013-11
Publisher
Elsevier Science B.V., Amsterdam.
Citation
BIOSENSORS & BIOELECTRONICS, Vol.49 No.- [2013] , p.415-419
Abstract
PSA (prostate-specific antigen) is one of the most widely used proteins for the diagnosis of breast and prostate cancer. Of note, PSA displays enzymatic activity for the specific peptide sequence HSSKLQ which it recognizes and cleaves. In this study, we developed a site-specific enzymatic-cleavage-reaction-based biosensor for the detection of PSA using fluorescein isothiocyanate (FITC)/peptide-conjugated gold (Au) nanoparticle complexes (FPANs). The FPANs do not initially fluoresce in the spectral region associated with the fluorophore, due to the quenching effect of the Au nanoparticles. When PSA was added to a solution containing the FPANs, PSA recognized and cleaved the specific sequence of the peptides attached to the Au nanoparticles. As a result, FITCs were separated from the Au nanoparticles and emitted strong fluorescence in their spectral region. Using this detection method, PSA was successfully detected as a function of concentration (10 pM-100 nM). This approach is superior to the immunoassay with respect to the performance of sensor, which is very rapid, simple, and one-step method for the detection of PSA and other protein markers can be measured for the early detection of several diseases. (C) 2013 The Author. Published by Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S095656631300376X?via%3Dihubhttp://hdl.handle.net/20.500.11754/46307
ISSN
0956-5663
DOI
10.1016/j.bios.2013.05.042
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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