Lobarstin Enhances Chemosensitivity in Human Glioblastoma T98G Cells
- Title
- Lobarstin Enhances Chemosensitivity in Human Glioblastoma T98G Cells
- Author
- 정희경
- Keywords
- Lobarstin; glioblastoma; temozolomide; chemosensitivity; DNA repair
- Issue Date
- 2013-12
- Publisher
- INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH
- Citation
- Anticancer Research, Vol.33, No.12 [2013], p5445-5452
- Abstract
- Background/Aim: Lobarstin is a metabolite occurring from the Antarctic lichen Stereocaulon alpnum. Human glioblastoma is highly resistant to chemotherapy with temozolomide. Lobarstin was examined for its effect on glioblastoma. Materials and Methods: Temozolomide-resistant T98G cells were subjected to toxicity test with temozolomide and/or lobarstin. DNA damage and recovery was assessed by the alkaline comet assay and expression of DNA repair genes was examined by RT-PCR and western blot analysis. Results: Lobarstin alone at 40 mu M was toxic against T98G, but had no effect in primary human fibroblasts. Co-treatment of lobarstin with temozolomide yielded enhanced toxicity. Temozolomide-alone or with lobarstin co-treatment gave similar extent of DNA damage. However, the recovery was reduced in co-treated cells. Expression of DNA repair genes, O-6-methylguanine-DNA methyltransferase, poly(ADPribose) polymerase I and ligase 3 were reduced in lobarstin-treated cells. Conclusion: Enhanced sensitivity to temozolomide by lobarstin co-treatment may be attributed to reduced DNA repair.
- URI
- http://ar.iiarjournals.org/content/33/12/5445.shorthttp://hdl.handle.net/20.500.11754/45965
- ISSN
- 0250-7005
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML