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Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro

Title
Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro
Author
이민형
Keywords
Dendrimer; Dexamethasone; Cationic Liposome; Gene Transfection
Issue Date
2011-02
Publisher
AMER SCIENTIFIC PUBLISHERS
Citation
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, Feb 2011, 11(2), P.1799-1802, 4P.
Abstract
In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. To make a liposomal solution for gene delivery, DOPE was used as a fusogenic helper lipid. In gel retardation assay, PAMAM-dexamethasone conjugate (PAM-Dex)/DOPE liposome/DNA complex was completely retarded at 8:1 N/P (nitrogen/phosphate) ratio. The physicochemical characteristics are studied by measuring the average size distribution and zeta-potential values of the complexes. In vitro transfection assay showed that the PAM-Dex/DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/DOPE liposome showed the lowest toxicity compared to PAMAM, PEI 25 kD and Lipofectamine. These results indicate that PAM-Dex/DOPE liposome has a potential to be used as an efficient gene carrier for gene therapy.
URI
http://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000002/art00178;jsessionid=6kdiumqrqae24.x-ic-live-01http://hdl.handle.net/20.500.11754/45913
ISSN
1533-4880; 1533-4899
DOI
10.1166/jnn.2011.3407
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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