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High Cholesterol Diet Induces IL-1 beta Expression in Adult but Not Larval Zebrafish

Title
High Cholesterol Diet Induces IL-1 beta Expression in Adult but Not Larval Zebrafish
Author
최재훈
Keywords
Animals; Blood Vessels; drug effects; immunology; metabolism; Cholesterol, adverse effects; Diet; High-Fat; Female; Gene Expression Regulation; Hypercholesterolemia; etiology, genetics; Immunity; Innate; Interleukin-1beta; Larva, Leukocytes; Male; Mice; Time Factors; Tumor Necrosis Factor-alpha; Zebrafish; Zebrafish Proteins
Issue Date
2013-06
Publisher
National Institutes of Health
Citation
Plos one, 2013, 8(6), P.e66970
Abstract
Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regarding inflammatory cytokine expression during the lipid accumulation in zebrafish larva and adult fish. In this study, we first demonstrated the expression levels of IL-1 beta and TNF-alpha in high cholesterol diet (HCD)-fed zebrafish larvae, and found that although HCD induced vascular lipid accumulation, the cytokine expressions in the larvae were not changed by HCD. Furthermore, there was no significant difference in leukocyte accumulation in vessels between control and HCD fed group. But prolonged HCD induced IL-1 beta expression in spleen and liver compared to those of control zebrafish, and produced very early stage of fatty streak lesion in dorsal aorta of 19 week HCD-fed zebrafish. These results indicate that HCD induced hypercholesterolemia and atherosclerotic changes in zebrafish are very early stage, and suggest the necessity of the generation of mutant zebrafish having a disruption in a lipid metabolism-related gene leading to severe hypercholesterolemia and advanced atherosclerosis.
URI
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0066970http://hdl.handle.net/20.500.11754/45389
ISSN
1932-6203
DOI
10.1371/journal.pone.0066970
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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