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Charting Microbial Phenotypes in Multiplex Nanoliter Batch Bioreactors

Title
Charting Microbial Phenotypes in Multiplex Nanoliter Batch Bioreactors
Author
Jong Wook Hong
Keywords
MICROFLUIDIC CELL-CULTURE; HIGH-THROUGHPUT; ESCHERICHIA-COLI; SOFT LITHOGRAPHY; MICROBIOREACTOR; CHIP; REORGANIZATION; MICROCHEMOSTAT
Issue Date
2013-04
Publisher
AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Citation
ANALYTICAL CHEMISTRY 권: 85 호: 12 페이지: 5892-5899
Abstract
High-throughput growth phenotyping is receiving great attention for establishing the genotype-phenotype map of sequenced organisms owing to the ready availability of complete genome sequences. To date, microbial growth phenotypes have been investigated mostly by the conventional method of batch cultivation using test tubes, Erlenmeyer flasks, or the recently available microwell plates. However, the current batch cultivation methods are time- and labor-intensive and often fail to consider sophisticated environmental changes. The implementation of batch cultures at the nanoliter scale has been difficult because of the quick evaporation of the culture medium inside the reactors. Here, we report a microfluidic system that allows independent cell cultures in evaporation-free multiplex nanoliter reactors under different culture conditions to assess the behavior of cells. The design allows three experimental replicates for each of eight culture environments in a single run. We demonstrate the versatility of the device by performing growth curve experiments with Escherichia coli and microbiological assays of antibiotics against the opportunistic pathogen Pseudomonas aeruginosa. Our study highlights that the microfluidic system can effectively replace the traditional batch culture methods with nanoliter volumes of bacterial cultivations, and it may be therefore promising for high-throughput growth phenotyping as well as for single-cell analyses.
Description
Global Frontier Intelligent Synthetic Biology Center
URI
http://dx.doi.org/10.1021/ac400648zhttp://hdl.handle.net/20.500.11754/44981
ISSN
0003-2700
DOI
10.1021/ac400648z
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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