Previously, mathematical modeling of a proton exchange membrane fuel cell has failed to precisely predict the performance in low relative-humidity operations. Herein, we report the fit parameters for water-uptake isotherm of the catalyst layer based on experimental measurements of dynamic vapor-sorption (DVS) technique. From the DVS measurement, it is revealed that the Nafion ionomer in the catalyst layer holds approximately 2.94 times lower water uptake than the Nafion membrane. By integrating this relation to the macroscopic model, the performance decrease due to the anode dehydration is appropriately captured relative to the previous model. Influences of the relative humidity on cell performance have been further investigated to correct the misguided prediction from the previous model.