Electrical Graphene Aptasensor for Ultra-Sensitive Detection of Anthrax Toxin with Amplified Signal Transduction
- Title
- Electrical Graphene Aptasensor for Ultra-Sensitive Detection of Anthrax Toxin with Amplified Signal Transduction
- Author
- 윤문영
- Keywords
- graphene; field-effect transistors; biosensors; aptamers; anthrax toxin; FIELD-EFFECT TRANSISTORS; PROTECTIVE ANTIGEN; BACILLUS-ANTHRACIS; OXIDE; BIOSENSORS; SENSORS; PROTEIN; LAYER; DNA
- Issue Date
- 2013-10
- Publisher
- John Wiley & Sons, Ltd
- Citation
- Small, 2013, 9(19), P.3352?3360
- Abstract
- Detection of the anthrax toxin, the protective antigen (PA), at the attomolar (aM) level is demonstrated by an electrical aptamer sensor based on a chemically derived graphene field-effect transistor (FET) platform. Higher affinity of the aptamer probes to PA in the aptamer-immobilized FET enables significant improvements in the limit of detection (LOD), dynamic range, and sensitivity compared to the antibody-immobilized FET. Transduction signal enhancement in the aptamer FET due to an increase in captured PA molecules results in a larger 30 mV/decade shift in the charge neutrality point (V-g,V-min) as a sensitivity parameter, with the dynamic range of the PA concentration between 12 aM (LOD) and 120 fM. An additional signal enhancement is obtained by the secondary aptamer-conjugated gold nanoparticles (AuNPs-aptamer), which have a sandwich structure of aptamer/PA/aptamer-AuNPs, induce an increase in charge-doping in the graphene channel, resulting in a reduction of the LOD to 1.2 aM with a three-fold increase in the V-g,V-min shift.
- URI
- http://onlinelibrary.wiley.com/doi/10.1002/smll.201203245/fullhttp://hdl.handle.net/20.500.11754/44882
- ISSN
- 1613-6810; 1613-6829
- DOI
- 10.1002/smll.201203245
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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