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Post-translational regulation of a hypoxia-responsive VEGF plasmid for the treatment of myocardial ischemia

Title
Post-translational regulation of a hypoxia-responsive VEGF plasmid for the treatment of myocardial ischemia
Author
이민형
Keywords
Myocardial infarct; Gene therapy; VEGFPAM-ABP
Issue Date
2013-05
Publisher
Elsevier LTD
Citation
Biomaterials, 2013, 34(26), P.6229-6238
Abstract
Vascular endothelial growth factor (VEGF) gene therapy to promote therapeutic angiogenesis has been advanced as an alternative treatment for myocardial ischemia. The unregulated expression of VEGF and the use of viral vectors, however, have slowed the clinical development of angiogenic gene therapy. The development of clinically beneficial angiogenic gene therapy requires a disease-specific gene expression system and an efficient non-viral gene carrier. To address these requirements, we developed a new post-translationally regulated hypoxia-responsible VEGF plasmid, pβ-SP-ODD-VEGF, and a dendrimer-type bio-reducible polymer, PAM-ABP. The efficacy of VEGF gene therapy with the PAM-ABP/pβ-SP-ODD-VEGF was evaluated and compared to the RTP-VEGF plasmid, a previously constructed hypoxia-inducible plasmid, in an ischemia/reperfusion (I/R) rat model. Cine magnetic resonance imaging was used to analyze the ischemia/reperfusion rats treated with either the PAM-ABP/pβ-SP-ODD-VEGF or the PAM-ABP/RTP-VEGF. The PAM-ABP/pβ-SP-ODD-VEGF treatment more effectively protected cardiomyocytes against apoptosis, preserved left ventricular (LV) function, and prevented LV remodeling compared to the PAM-ABP/RTP-VEGF-treated rats. These results suggest that the pβ-SP-ODD-VEGF with PAM-ABP may be efficacious in the treatment of acute ischemic heart disease.
URI
https://www.sciencedirect.com/science/article/pii/S0142961213005358?via%3Dihubhttp://hdl.handle.net/20.500.11754/44752
ISSN
0142-9612
DOI
10.1016/j.biomaterials.2013.04.061
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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