Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-03-09T07:20:38Z | - |
dc.date.available | 2018-03-09T07:20:38Z | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | BIOMATERIALS, 권: 34, 호: 30, 페이지: 7453-7461 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0142961213006741?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/44392 | - |
dc.description.abstract | Inhalation of airborne particulate matter (PM), such as silicon dioxide (SiO2) and titanium dioxide (TiO2), induces acute lung inflammation. siRNA therapy has been proposed as a method to repair acute lung inflammation. To determine whether DEXA-PEI/MIF siRNA contributes to SiO2-induced acute lung inflammation repair, we administered Dexa-PEI/MIF siRNA in SiO2-treated Beas-2b cells and instilled DEXA-PEI-MIF siRNA intratracheally in mice with SiO2-induced acute lung inflammation. Using genetic (MIF mRNA RT-PCR), histological (H&E and PAS) and immunohistochemical (MIF and Muc5ac) analyses, we estimated the acute lung inflammation in Beas-2b cells and BALB/c mice. Cells and mice treated with SiO2 particles demonstrated pulmonary inflammation. DEXA-PEI/MIF siRNA restricted the extent of the pulmonary inflammation reaction to SiO2 in cells and mice. In case of SiO2-treated Beas-2b cells, only DEXA-PEI treatment failed to effectively regulate MIF mRNA release. At the same time, only DEXA-PEI treatment adjusted the amount of MIF mRNA to some extent in SiO2-treated BALB/c mice. siRNA treatment did not markedly control MIF mRNA release in mice. We also observed that the amount of MIF mRNA was decreased in cells and mice treated with DEXA-PEI/MIF siRNA. The increase of MIF mRNA markedly increased Muc5ac; in contrast, the decrease of MIF mRNA using DEXA-PEI/MIF siRNA effectively lowered Muc5ac in SiO2-treated cells and mice. These results suggest that DEXA-PEI plays a role in delivering siRNA to the nucleus as a carrier and limits the extent of acute lung inflammation. MIF siRNA also contributed to the reparative lung response in SiO2-induced pulmonary inflammation. (C) 2013 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0016846). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | PM (particulate matter) | en_US |
dc.subject | Airway inflammation | en_US |
dc.subject | DEXA-PEI | en_US |
dc.subject | MIF | en_US |
dc.subject | siRNA | en_US |
dc.subject | Gene delivery | en_US |
dc.title | Dexamethasone-conjugated polyethylenimine/MIF siRNA complex regulation of particulate matter-induced airway inflammation | en_US |
dc.title.alternative | MIF siRNA complex regulation of particulate matter-induced airway inflammation | en_US |
dc.type | Article | en_US |
dc.relation.volume | 34 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.05.082 | - |
dc.relation.page | 7453-7461 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Choi, Moonhwan | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Rhim, Taiyoun | - |
dc.relation.code | 2013009154 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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