85 0

Inhibitory effects of eupatilin on tumor invasion of human gastric cancer MKN-1 cells

Title
Inhibitory effects of eupatilin on tumor invasion of human gastric cancer MKN-1 cells
Author
박병배
Keywords
NECROSIS-FACTOR-ALPHA; NF-KAPPA-B; HELICOBACTER-PYLORI INFECTION; BETA-CATENIN; EPITHELIAL-CELLS; UROKINASE RECEPTOR; ARTEMISIA PLANTS; GENE-EXPRESSION; UP-REGULATION; AGS CELLS
Issue Date
2013-04
Publisher
Springer
Citation
Tumor Biology, April 2013, 34(2), P.875-885
Abstract
Extracts of the whole herb of Artemisia asiatica Nakai (Asteraceae) are used in traditional oriental medicine to treat inflammation. Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is one of the pharmacologically active components found in A. asiatica, and has been shown to possess anti-tumoral effects in some malignancies, including gastric cancer. However, its anti-metastatic effect in gastric cancer is hardly known. In this study, anti-metastatic effect of eupatilin was investigated in the human gastric cancer cell line, MKN-1. Eupatilin inhibited MKN-1 growth in a dose- and a time-dependent manner, and induced apoptosis with a concomitant increase of caspase-3 activity. ELISA demonstrated that release of pro-inflammatory cytokines (IL-1 beta, TNF-alpha, IL-6, and IL-8) was significantly reduced by eupatilin. And p-AKT and p-ERK (p44/42) was reduced. Expression level of beta-catenin and integrin was reduced and p-GSK beta was increased. In transcription reporter system, the activity of the transcriptional factor, NF-kappa B, was reduced by eupatilin and the expression of p65 was down-regulated when MKN-1 cells were treated with eupatilin. Moreover, a zymography study revealed that this reduction in invasive potential resulted from a reduction in type IV collagenolytic (gelatinolytic) activity. The expressions of metalloproteinases (MMP-2 and MMP-9) were also reduced in MKN-1 cells treated with eupatilin. In vitro invasion assay, eupatilin inhibited MKN-1 penetrating reconstituted basement membrane barriers. These results suggest that eupatilin inhibits the MKN-1 gastric cancer cell proliferation via activation of caspase-3 and the metastatic potential of gastric cancer cells via down-regulation of NF-kappa B activity followed by reduction of pro-inflammatory cytokine-mediated MMPs expressions.
URI
https://link.springer.com/article/10.1007%2Fs13277-012-0621-yhttp://hdl.handle.net/20.500.11754/44347
ISSN
1010-4283
DOI
10.1007/s13277-012-0621-y
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE