Degradome sequencing reveals an endogenous microRNA target in C. elegans
- Title
- Degradome sequencing reveals an endogenous microRNA target in C. elegans
- Author
- 남진우
- Keywords
- MicroRNA; Degradome sequencing; MicroRNA-directed cleavage; C. elegans
- Issue Date
- 2013-04
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- FEBS LETTERS, April 2013, 587(7), P.964-969
- Abstract
- Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3' untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR-249. These findings may lead to a better understanding of miRNA-mediated gene regulation in nematodes. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- URI
- http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2013.02.029/abstract;jsessionid=98A05291D318A246A1C8DD7593563F39.f01t03http://hdl.handle.net/20.500.11754/44217
- ISSN
- 0014-5793
- DOI
- 10.1016/j.febslet.2013.02.029
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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