48 0

Degradome sequencing reveals an endogenous microRNA target in C. elegans

Title
Degradome sequencing reveals an endogenous microRNA target in C. elegans
Author
남진우
Keywords
MicroRNA; Degradome sequencing; MicroRNA-directed cleavage; C. elegans
Issue Date
2013-04
Publisher
ELSEVIER SCIENCE BV
Citation
FEBS LETTERS, April 2013, 587(7), P.964-969
Abstract
Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3' untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR-249. These findings may lead to a better understanding of miRNA-mediated gene regulation in nematodes. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
URI
http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2013.02.029/abstract;jsessionid=98A05291D318A246A1C8DD7593563F39.f01t03http://hdl.handle.net/20.500.11754/44217
ISSN
0014-5793
DOI
10.1016/j.febslet.2013.02.029
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE