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Lipoic Acid Prevents the Changes of Intracellular Lipid Partitioning by Free Fatty Acid

Title
Lipoic Acid Prevents the Changes of Intracellular Lipid Partitioning by Free Fatty Acid
Author
전대원
Keywords
Thioctic acid; Lipotoxicity; Lipid partitioning; Non-alcoholic steatohepatitis; Liver cirrhosis
Issue Date
2013-03
Publisher
EDITORIAL OFFICE GUT & LIVER, 305 LOTTE GOLD ROSE II, 890-59, DAECHI 4-DONG, GANGNAM-GU, SEOUL, 135-839, SOUTH KOREA
Citation
GUT AND LIVER, 권: 7 호: 2, 페이지: 221-227
Abstract
Background/Aims: It is suggested that the hepatic lipid composition is more important than lipid quantity in the pathogenesis of non-alcoholic steatohepatitis. We examined whether lipoic acid (LA) could alter intrahepatic lipid composition and free cholesterol distribution. Methods: HepG2 cells were cultured with palmitic acid (PA) with and without LA. Apoptosis, changes of the mitochondrial structure, intracellular lipid partitioning, and reactive oxygen species (ROS) activity were measured. Results: Free fatty acid (FA) increased apoptosis, and LA co-treatment prevented this lipotoxicity (apoptosis in controls vs PA vs PA+LA, 0.5% vs 19.5% vs 1.6%, p<0.05). LA also restored the intracellular mitochondrial DNA copy number (553±33.8 copies vs 291±14.55 copies vs 421±21.05 copies, p<0.05) and reversed the morphological changes induced by PA. In addition, ROS was increased in response to PA and was decreased in response to LA co-treatment (41,382 relative fluorescence unit [RFU] vs 43,646 RFU vs 41,935 RFU, p<0.05). LA co-treatment increased the monounsaturated and polyunsaturated FA concentrations and decreased the total saturated FA fraction. It also prevented the movement of intracellular free cholesterol from the cell membrane to the cytoplasm. Conclusions: LA opposes free FA-generated lipotoxicity by altering the intracellular lipid composition and free cholesterol distribution. (Gut Liver 2013;7:221-227)
URI
http://www.gutnliver.org/journal/view.html?volume=7&number=2&spage=221&year=2013http://hdl.handle.net/20.500.11754/44120
ISSN
1976-2283
DOI
10.5009/gnl.2013.7.2.221
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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