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FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols

Title
FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols
Other Titles
Ag hybrid nanoparticle for selective determination of molecular biothiols
Author
주재범
Keywords
Molecular biothiols; Chromogenic detection; Silver-coated magnetic nanoparticles; Fluorescein isothiocyanate; Living cells
Issue Date
2014-03
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Sensors and actuators. B Chemical, Vol.193 No.- [2014], 857-863
Abstract
A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core?shell Fe3O4/Ag hybrid nanoparticles. Ag coated magnetic Fe3O4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core?shell nanoparticles by Ag?SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the AgS bond is stronger than Ag?SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence “off-on” probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10nM and 20nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems.
URI
http://www.sciencedirect.com/science/article/pii/S0925400513015268?via%3Dihubhttp://hdl.handle.net/20.500.11754/44108
ISSN
0925-4005
DOI
10.1016/j.snb.2013.12.053
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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