60 0

CD24 regulates cell proliferation and transforming growth factor beta-induced epithelial to mesenchymal transition through modulation of integrin beta 1 stability

Title
CD24 regulates cell proliferation and transforming growth factor beta-induced epithelial to mesenchymal transition through modulation of integrin beta 1 stability
Author
신인철
Keywords
CD24; EMT; ERK; FAK; Integrin; FOCAL ADHESION KINASE; BREAST-CANCER CELLS; INDEPENDENT PROGNOSTIC MARKER; TGF-BETA; C-SRC; SIGNALING PATHWAYS; PROTEIN-KINASE; IN-VITRO; B-CELLS; EXPRESSION
Issue Date
2012-11
Publisher
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Citation
CELLULAR SIGNALLING, Vol.24, No.11 [2012], p2132-2142
Abstract
To determine the role of CD24 in breast cancer cells, we knocked down CD24 in MCF-7 human breast cancer cells by retroviral delivery of shRNA. MCF-7 cells with knocked down CD24 (MCF-7 hCD24 shRNA) exhibited decreased cell proliferation and cell adhesion as compared to control MCF-7 mCD24 shRNA cells. Decreased proliferation of MCF-7 hCD24 shRNA cells resulted from the inhibition of cell cycle progression from G1 to S phase. The specific inhibition of MEK/ERK signaling by CD24 ablation might be responsible for the inhibition of cell proliferation. Phosphorylation of Src/FAK and TGF-beta 1 -mediated epithelial to mesenchymal transition was also down-regulated in MCF-7 hCD24 shRNA cells. Reduced Src/FAK activity was caused by a decrease in integrin beta 1 bound with CD24 and subsequent destabilization of integrin beta 1. Our results suggest that down-regulation of Raf/MEK/ERK signaling via Src/FAK may be dependent on integrin beta 1 function and that this mechanism is largely responsible for the CD24 ablation-induced decreases in cell proliferation and epithelial to mesenchymal transition. (C) 2012 Elsevier Inc. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0898656812001957?via%3Dihub
ISSN
0898-6568
DOI
10.1016/j.cellsig.2012.07.005
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE