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Comparative analysis of virulence factors secreted by Bacillus anthracis Sterne at host body temperature

Title
Comparative analysis of virulence factors secreted by Bacillus anthracis Sterne at host body temperature
Author
채영규
Keywords
Bacillus anthracis; Host body temperature; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Secretome; Two-dimensional gel electrophoresis; Virulence factor
Issue Date
2012-04
Publisher
WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
Citation
LETTERS IN APPLIED MICROBIOLOGY, 권: 54, 호: 4, 페이지: 306-312
Abstract
Aims: For the analysis of virulence factors produced and secreted by Bacillus anthracis vegetative cells during mammalian host infection, we evaluated the secretome of B. anthracis Sterne exposed to host-specific factors specifically to host body temperature. Methods and Results: We employed a comparative proteomics-based appro 00000418 ach to analyse the proteins secreted by B. anthracis Sterne under host-specific body temperature conditions. A total of 17 proteins encoded on a single chromosome and the pXO1 plasmid were identified by peptide mass fingerprinting. Multiple algorithms were used to predict the secretion mechanisms of the detected proteins in B. anthracis. Conclusions: Several putative virulence factors and known factors responsible for sporulation were differentially regulated, including CodY, pXO1-130 and BA1952, revealing insights into temperature cues in the B. anthracis secretome. Significance and Impact of the Study: This study identified temperature-regulated proteins. Further studies aimed at understanding the physical and functional roles of these proteins in infection and control by elevated temperatures will contribute to detection, diagnostics and prophylaxis.
URI
http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2012.03209.x/abstract;jsessionid=CC9B42B6E183D0B6DC8F10A52C4E3C2C.f04t02http://hdl.handle.net/20.500.11754/42721
ISSN
0266-8254; 1472-765X
DOI
10.1111/j.1472-765X.2012.03209.x
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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