Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-03-01T02:05:42Z | - |
dc.date.available | 2018-03-01T02:05:42Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Journal of Andrology, Sep-Oct 2012, 33(5), P.845-853, 9p. | en_US |
dc.identifier.issn | 0196-3635 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.2164/jandrol.111.016014 | - |
dc.description.abstract | Cavernous hypoxia is an important factor in the pathogenesis of vasculogenic erectile dysfunction (ED). Therefore, the hypoxia-inducible gene expression system can be exploited as gene therapy for vasculogenic ED. This study was undertaken to examine the effectiveness of a hypoxia-inducible gene expression system, namely, the RTP801 promoter or the erythropoietin enhancer, in a mouse model of hypercholesterolemic ED in vivo and in primary cultured mouse cavernous endothelial cells in vitro. Two-month-old male C57BL/6 mice were fed a diet containing 4% cholesterol and 1% cholic acid, and age-matched control animals were fed a normal diet for 3 months. Mouse cavernous endothelial cells were isolated and cultured under normoxic or hypoxic conditions. After treatment of animals or endothelial cells with pSV-Luc, pRTP801-Luc, or pEpo-SV-Luc vector, gene expression was evaluated by luciferase assay, and the gene expression area was evaluated by immunohistochemistry. Plasmids pRTP801-Luc and pEpo-SV-Luc induced gene expression significantly in the hypercholesterolemic mice and in cavernous endothelial cells under hypoxia, and the highest gene expression was noted in the group treated with pEpo-SV-Luc. Gene expression was higher for more than 7 days in the hypercholesterolemic mice injected with pEpo-SV-Luc than in mice injected with pSV-Luc. As shown by immunohistochemistry, the gene expression area was also greater in the pEpo-SV-Luc group than in the pSV-Luc group, but the difference was not as great as that in luciferase activity. The hypoxia-specific gene expression system could be a valuable tool for facilitating gene delivery into ischemic corpus cavernosum tissue resulting from vascular causes. | en_US |
dc.description.sponsorship | Supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare, and Family Affairs, Republic of Korea (A084511. J.-K.S.). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.subject | Hypoxia response element | en_US |
dc.subject | hypercholesterolemia | en_US |
dc.subject | vector | en_US |
dc.title | Gene Therapy With an Erythropoietin Enhancer-Mediated, Hypoxia-Inducible Gene Expression System in the Corpus Cavernosum of Mice With High-Cholesterol Diet-Induced Erectile Dysfunction | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 10.2164/jandrol.111.016014 | - |
dc.relation.page | 845-853 | - |
dc.relation.journal | JOURNAL OF ANDROLOGY | - |
dc.contributor.googleauthor | Ryu, Ji-Kan | - |
dc.contributor.googleauthor | Choi, Min Ji | - |
dc.contributor.googleauthor | Jin, Hai-Rong | - |
dc.contributor.googleauthor | Kim, Woo Jean | - |
dc.contributor.googleauthor | Yin, Guo Nan | - |
dc.contributor.googleauthor | Song, Kang-Moon | - |
dc.contributor.googleauthor | Kwon, Mi-Hye | - |
dc.contributor.googleauthor | Suh, Jun-Kyu | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Kim, Hyun Ah | - |
dc.contributor.googleauthor | 류기간 | - |
dc.contributor.googleauthor | 최민지 | - |
dc.contributor.googleauthor | 진해롱 | - |
dc.contributor.googleauthor | 김우진 | - |
dc.contributor.googleauthor | 인교난 | - |
dc.contributor.googleauthor | 송강문 | - |
dc.contributor.googleauthor | 권미혜 | - |
dc.contributor.googleauthor | 서준규 | - |
dc.contributor.googleauthor | 이민형 | - |
dc.contributor.googleauthor | 김현아 | - |
dc.relation.code | 2012204631 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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