Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-03-01T02:04:11Z | - |
dc.date.available | 2018-03-01T02:04:11Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Biomaterials, Sep 2012, 33(27), P.6542-6550 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S014296121200587X?via%3Dihub | - |
dc.description.abstract | In this study, the R7L10 peptide, which is composed of a 7-arginine stretch and a 10-leucine stretch, was evaluated as a carrier for the combined delivery of curcumin and plasmid DNA (pDNA) into the lungs. Curcumin is a natural product with anti-inflammatory and anti-tumor effects. Curcumin-loaded R7L10 (R7L10-curucmin) was prepared by an oil-in-water (O/W) emulsion/solvent evaporation method. In vitro transfection showed that R7L10-curcumin had higher transfection efficiency than R7L10. Although R7L10-curcumin had lower transfection efficiency than polyethylenimine (25 kDa, PEI25k) and lipofectamine, R7L10-curcumin had lower cytotoxicity. In gel retardation assays and heparin competition assays, R7L10-curcumin formed a more stable complex with pDNA than R7L10. The intracellular curcumin delivery efficiency of R7L10-curcumin was higher than that of curcumin only. Furthermore, R7L10-curcumin more efficiently decreased TNF-alpha level in lipopolysaccharide (LPS)-activated Raw264.7 macrophage cells than curcumin only. For in vivo evaluation, pDNA/R7L10-curcumin complexes were administered into mouse lungs by intratracheal instillation. The results revealed that R7L10-curcumin delivered pDNA more efficiently than R7L10, poly-L-lysine (PLL), or PEI25k. In addition, R7L10-curcumin decreased TNF-alpha level in lung tissues in an acute lung injury mouse model. In contrast to PEI25k, R7L10-curcumin did not show liver toxicity after intravenous injection. These results suggest that R7L10-curcumin is a useful carrier for the combined delivery of curcumin and pDNA into the lungs. (c) 2012 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by a grant from the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2011K000803, 20110026013). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science BV | en_US |
dc.subject | DNA | en_US |
dc.subject | Gene transfer | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Cell viability | en_US |
dc.subject | Inflammation | en_US |
dc.title | Amphiphilic peptide carrier for the combined delivery of curcumin and plasmid DNA into the lungs | en_US |
dc.type | Article | en_US |
dc.relation.no | 27 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 0.1016/j.biomaterials.2012.05.046 | - |
dc.relation.page | 6542-6550 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Park, Ji Hwan | - |
dc.contributor.googleauthor | Kim, Hyun Ah | - |
dc.contributor.googleauthor | Park, Jin Hyeong | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | 박지환 | - |
dc.contributor.googleauthor | 김현아 | - |
dc.contributor.googleauthor | 박지형 | - |
dc.contributor.googleauthor | 이민형 | - |
dc.relation.code | 2012201314 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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