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Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in Caenorhabditis elegans

Title
Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in Caenorhabditis elegans
Author
안주홍
Keywords
C. elegans; longevity; oxidative stress; thioredoxin reductase; V-ATPase; C-ELEGANS; OXIDATIVE STRESS; REDOX REGULATION; V-ATPASE; SELENOCYSTEINE; MITOCHONDRIAL; GLUTATHIONE; INHIBITION; INSERTION; SUBUNIT
Issue Date
2012-08
Publisher
한국분자세포생물학회
Citation
Molecules and cells,Vol.34,No.2 [2012],209-218(10쪽)
Abstract
Thioredoxin reductase (TrxR) is a member of the pyridine nucleotide-disulfide reductase family, which mainly func-tions in the thioredoxin system. TrxR is found in all living organisms and exists in two major ubiquitous isoen-zymes in higher eukaryotic cells; One is cytosolic and the other mitochondrial. Mitochondrial TrxR functions to protect mitochondria from oxidative stress, where reactive oxidative species are mainly generated, while cytosolic TrxR plays a role to maintain optimal oxido-reductive status in cytosol. In this study, we report differential physiological functions of these two TrxRs in C. elegans. trxr-1, the cytosolic TrxR, is highly expressed in pharynx, vulva and intestine, whereas trxr-2, the mitochondrial TrxR, is mainly expressed in pharyngeal and body wall muscles. Deficiency of the non-selenoprotein trxr-2 caused defects in longevity and delayed development under stress conditions, while deletion mutation of the selenoprotein trxr-1 resulted in interference in acidification of lysosomal compartment in intestine. Interestingly, the acidification defect of trxr-1(jh143) deletion mutant was rescued, not only by selenocystein-containing wild type TRXR-1, but also cysteine-substituted mutant TRXR-1. Both trxr-1 and trxr-2 were up-regulated when worms were challenged by environmental stress such as heat shock. These results suggest that trxr-1 and trxr-2 function differently at organismal level presumably by their differential sub-cellular localization in C. elegans.
URI
https://link.springer.com/article/10.1007%2Fs10059-012-0155-6http://hdl.handle.net/20.500.11754/41327
ISSN
1016-8478
DOI
10.1007/s10059-012-0155-6
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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