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dc.contributor.author황승용-
dc.date.accessioned2018-02-28T05:44:04Z-
dc.date.available2018-02-28T05:44:04Z-
dc.date.issued2011-05-
dc.identifier.citationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,11(5), 4250-4253en_US
dc.identifier.issn1533-4880-
dc.identifier.urihttp://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000005/art00075-
dc.description.abstractIn this paper, we describe the design and fabrication of a microfluidic device for cell lysis and DNA purification, and the results of device tests using a real sample of buccal cells. Cell lysis was thermally executed for two minutes at 80 degrees C in a serpentine type microreactor (20 mu L) using an Au microheater with a microsensor. The DNA was then mixed with other residual products and purified by a new filtration process involving micropillars and 50-80 mu m microbeads. The entire process of sample loading, cell lysis, DNA purification, and sample extraction was successfully completed in the microchip within five minutes. Sample preparation within the microchip was verified by performing a SY158 gene PCR analysis and gel electrophoresis on the products obtained from the chip. The new purification method enhanced DNA purity from 0.93 to 1.62 after purification.en_US
dc.language.isoenen_US
dc.publisherAMER SCIENTIFIC PUBLISHERSen_US
dc.subjectMicrofluidic Deviceen_US
dc.subjectCell Lysisen_US
dc.subjectDNA Purificationen_US
dc.titleAn Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samplesen_US
dc.typeArticleen_US
dc.relation.no5-
dc.relation.volume11-
dc.identifier.doi10.1166/jnn.2011.3640-
dc.relation.page4250-4253-
dc.relation.journalJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY-
dc.contributor.googleauthorHa, Seung-Mo-
dc.contributor.googleauthorCho, Woong-
dc.contributor.googleauthorAhn, Yoomin-
dc.contributor.googleauthorHwang, Seung Yong-
dc.relation.code2011214452-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidsyhwang-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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