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An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples

Title
An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples
Author
황승용
Keywords
Microfluidic Device; Cell Lysis; DNA Purification
Issue Date
2011-05
Publisher
AMER SCIENTIFIC PUBLISHERS
Citation
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,11(5), 4250-4253
Abstract
In this paper, we describe the design and fabrication of a microfluidic device for cell lysis and DNA purification, and the results of device tests using a real sample of buccal cells. Cell lysis was thermally executed for two minutes at 80 degrees C in a serpentine type microreactor (20 mu L) using an Au microheater with a microsensor. The DNA was then mixed with other residual products and purified by a new filtration process involving micropillars and 50-80 mu m microbeads. The entire process of sample loading, cell lysis, DNA purification, and sample extraction was successfully completed in the microchip within five minutes. Sample preparation within the microchip was verified by performing a SY158 gene PCR analysis and gel electrophoresis on the products obtained from the chip. The new purification method enhanced DNA purity from 0.93 to 1.62 after purification.
URI
http://www.ingentaconnect.com/content/asp/jnn/2011/00000011/00000005/art00075
ISSN
1533-4880
DOI
10.1166/jnn.2011.3640
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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