Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이동윤 | - |
dc.date.accessioned | 2018-02-28T05:23:05Z | - |
dc.date.available | 2018-02-28T05:23:05Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Journal of Drug Targeting, Sep 2012, 20(8), P667-677 | en_US |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://www.tandfonline.com/doi/abs/10.3109/1061186X.2012.712127?journalCode=idrt20 | - |
dc.description.abstract | Polyamidoamine (PAM) dendrimers with low generation such as PAM generation 1 (PAMG1) and PAM generation 2 (PAMG2) have been widely used as a gene carrier due to low toxicity, albeit their low transfection efficiency. In this study, dexamethasone was conjugated to PAMG1 and PAMG2 in order to increase the transfection efficiency. In a gel retardation assay, the dexamethasone conjugated PAMG1 and PAMG2 (PAMG1-Dexa and PAMG2-Dexa) retarded plasmid DNA (pDNA) completely at 5:1 and 3:1 weight ratios (polymer:pDNA), respectively. In transfection assays, PAMG1-Dexa and PAMG2-Dexa had the highest transfection efficiency at 20:1 and 10:1 weight ratios, respectively. In addition, PAMG1-Dexa and PAMG2-Dexa had higher transfection efficiencies than PAMG1, PAMG2, PEI25k, and lipofectamine. In a MTT assay, PAMG1-Dexa and PAMG2-Dexa were less cytotoxic than lipofectamine. In addition, PAMG1-Dexa and PAMG2-Dexa decreased the TNF-a level more efficiently than dexamethasone only in the lipopolysaccharide (LPS)-induced Raw264.7 cells. Therefore, PAMG1-Dexa and PAMG2-Dexa may prove to be useful as gene delivery carriers with an anti-inflammatory effect. | en_US |
dc.description.sponsorship | This work was supported by a grant from the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2011K000803, 20110026013, 20110027308). The authors report no conflicts of interest. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Informa Healthcare | en_US |
dc.subject | Dexamethasone | en_US |
dc.subject | gene delivery | en_US |
dc.subject | polyamidoamine | en_US |
dc.subject | transfection | en_US |
dc.subject | tumor necrosis factor-alpha | en_US |
dc.title | Dexamethasone conjugation to polyamidoamine dendrimers G1 and G2 for enhanced transfection efficiency with an anti-inflammatory effect | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 20 | - |
dc.identifier.doi | 10.3109/1061186X.2012.712127 | - |
dc.relation.page | 667-677 | - |
dc.relation.journal | JOURNAL OF DRUG TARGETING | - |
dc.contributor.googleauthor | Kim, Jin Young | - |
dc.contributor.googleauthor | Ryu, Jae Hwan | - |
dc.contributor.googleauthor | Hyun, Hyesun | - |
dc.contributor.googleauthor | Kim, Hyun Ah | - |
dc.contributor.googleauthor | Choi, Joon Sig | - |
dc.contributor.googleauthor | Lee, Dong Yun | - |
dc.contributor.googleauthor | Rhim, Taiyoun | - |
dc.contributor.googleauthor | Park, Jeong Hyun | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | 김진영 | - |
dc.contributor.googleauthor | 류재환 | - |
dc.contributor.googleauthor | 현혜선 | - |
dc.contributor.googleauthor | 김현아 | - |
dc.contributor.googleauthor | 최준식 | - |
dc.contributor.googleauthor | 이동윤 | - |
dc.contributor.googleauthor | 박정현 | - |
dc.contributor.googleauthor | 이민정 | - |
dc.relation.code | 2012204972 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | dongyunlee | - |
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