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Heme oxygenase 1-mediated novel anti-inflammatory activities of Salvia plebeia and its active components

Title
Heme oxygenase 1-mediated novel anti-inflammatory activities of Salvia plebeia and its active components
Author
김철영
Keywords
Salvia plebeia R. Br; Anti-inflammatory activity; Heme oxygenase-1 (HO-1); Nuclear factor erythroid 2-related factor2 (Nrf2); Nepetin; Hispidulin; RAW 264.7 CELLS; ANTIOXIDANT ACTIVITY; MICE; EXTRACT; INFLAMMATION; INJURY; INVOLVEMENT; INDUCTION; R.BR.; HO-1
Issue Date
2015-11
Publisher
ELSEVIER IRELAND LTD
Citation
JOURNAL OF ETHNOPHARMACOLOGY, v. 174, Page. 322-330
Abstract
Ethnopharmacological relevance: Salvia plebeia R. Br. (SP) has been widely used as a traditional folk medicine for the treatment of infectious diseases and pain. An anti-inflammatory potential of SP has remains largely unknown. Aim of the study: We tried to elucidate the principle mechanism and the active ingredients underlying the anti-inflammatory activities of SP. Materials and methods: We investigated the protective activities of SP methanolic extract (SPME) and seven representative ingredients against inflammation. Quantitative analysis using HPLC-DAD-ESI/MS was conducted to determine the relative amounts of these seven active ingredients in SPME. Both in vitro murine macrophages and in vivo mouse models were employed to elucidate SP- and active ingredient-mediated anti-inflammatory effects. Results: SPME significantly reduced inflammatory processes both in vivo in a TPA-induced ear edema model and in vitro in lipopolysaccharide (LPS)-activated macrophages. SPME decreased the release of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) and expression of inducible nitric oxide synthase (iNOS). Seven active components (luteoloside (C1), nepitrin (C2), homoplantagenin (C3), luteolin (C4), nepetin (C5), hispidulin (C6), and eupatorin (C7)) of SPME were analyzed and their relative concentrations were determined, demonstrating that C2, C3, C5 and C6 were present in higher amounts than were Cl, C4, and C7. These major compounds inhibited NO and PGE(2) production, and iNOS and COX-II protein expression through heme oxygenase-1 (HO-1) induction via activation of nuclear factor erythroid 2 related factor2 (Nrf2). Conclusion: Our data demonstrate that SPME possesses potent in vitro and in vivo anti-inflammatory activities. Nepetin and hispidulin, and their glycosides are the major active compounds in SPME, and their effects are mediated by Nrf2/HO-1 signaling. Taken together, we propose that SPME and its active ingredients may serve as novel therapeutic candidates for diseases associated with excessive inflammation. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0378874115300969http://hdl.handle.net/20.500.11754/40972
ISSN
0378-8741
DOI
10.1016/j.jep.2015.08.028
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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