Inflammatory response of a prostate stromal cell line induced by Trichomonas vaginalis

Title
Inflammatory response of a prostate stromal cell line induced by Trichomonas vaginalis
Author
류재숙
Keywords
chemokine; inflammation; monocyte migration; neutrophil; prostate stromal cell; Trichomonas vaginalis
Issue Date
2016-04
Publisher
WILEY-BLACKWELL
Citation
PARASITE IMMUNOLOGY, v. 38, NO 4, Page. 218-227
Abstract
While Trichomonas vaginalis, a cause of sexually transmitted infection, is known as a surface-dwelling protozoa, trichomonads have been detected in prostatic tissue from benign prostatic hyperplasia and prostatitis by immunoperoxidase assay or PCR. However, the immune response of prostate stromal cells infected with T.vaginalis has not been investigated. Our objective was to investigate whether T.vaginalis could induce an inflammatory response in prostate stromal cells. Incubation of a human prostate stromal myofibroblast cells (WPMY-1) with live T.vaginalis T016 increased expression of the inflammatory chemokines CXCL8 and CCL2. In addition, TLR4, ROS, MAPK and NF-B expression increased, while inhibitors of TLR4, ROS, MAPKs and NF-B reduced CXCL8 and CCL2 production. Medium conditioned by incubation of WPMY-1 cells with T.vaginalis stimulated the migration of human neutrophils and monocytes (THP-1 cells). We conclude that T.vaginalis increases CXCL8 and CCL2 production by human prostate stromal cells by activating TLR4, ROS, MAPKs and NF-B, and this in turn attracts neutrophils and monocytes and leads to an inflammatory response. This study is the first attempt to demonstrate an inflammatory reaction in prostate stromal cells caused by T.vaginalis.
URI
http://onlinelibrary.wiley.com/doi/10.1111/pim.12308/abstract;jsessionid=76A5A6D8D6F9E76BBAA188BF6BA0247A.f02t04?systemMessage=Please+be+advised+that+we+experienced+an+unexpected+issue+that+occurred+on+Saturday+and+Sunday+January+20th+and+21st+that+caused+the+site+to+be+down+for+an+extended+period+of+time+and+affected+the+ability+of+users+to+access+content+on+Wiley+Online+Library.+This+issue+has+now+been+fully+resolved.++We+apologize+for+any+inconvenience+this+may+have+caused+and+are+working+to+ensure+that+we+can+alert+you+immediately+of+any+unplanned+periods+of+downtime+or+disruption+in+the+future.http://hdl.handle.net/20.500.11754/40961
ISSN
0141-9838; 1365-3024
DOI
10.1111/pim.12308
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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