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Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

Title
Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment
Author
김철영
Keywords
Abies koreana Wilson; centrifugal partition chromatography; DPPH center dot-HPLC experiment; dihydrokaempferol 7-O--D-glucopyranoside; maltol; trans-piceid; PURIFICATION
Issue Date
2015-11
Publisher
TAYLOR & FRANCIS INC
Citation
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v. 38, No. 18, Page. 1681-1686
Abstract
Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH center dot-HPLC experiment. In DPPH center dot-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3mg), dihydrokaempferol 7-O--D-glucopyranoside (230.4mg), and trans-piceid (165.9mg) were purified from 2.19g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by H-1, C-13 NMR, and ESI-MS.
URI
https://www.tandfonline.com/doi/abs/10.1080/10826076.2015.1091010http://hdl.handle.net/20.500.11754/40955
ISSN
1082-6076; 1520-572X
DOI
10.1080/10826076.2015.1091010
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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