The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal c-globin genes
- Title
- The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal c-globin genes
- Author
- 김철근
- Keywords
- Acetylation; Chromatin; chemistry; GATA1 Transcription Factor; genetics; metabolism; physiology; Gene Knockdown Techniques; Histones; Humans; K562 Cells; Locus Control Region; NF-E2 Transcription Factor; NF-E2 Transcription Factor; p45 Subunit; Transcription; Genetic; gamma-Globins
- Issue Date
- 2011-09
- Publisher
- MEDLINE/PubMed
- Citation
- Nucleic Acids Research, Vol.39, No.16 [2011], p6944-6955
- Abstract
- GATA-1 and NF-E2 are erythroid specific activators that bind to the β-globin locus. To explore the roles of these activators in transcription of the human fetal stage specific γ-globin genes, we reduced GATA-1 and p45/NF-E2 using shRNA in erythroid K562 cells. GATA-1 or p45/NF-E2 knockdown inhibited the transcription of the γ-globin genes, hypersensitive site (HS) formation in the LCR and chromatin loop formation of the β-globin locus, but histone acetylation across the locus was decreased only in the case of GATA-1 knockdown. In p45/NF-E2 knockdown cells, GATA-1 binding was maintained at the LCR HSs and γ-globin promoter, but NF-E2 binding at the LCR HSs was reduced by GATA-1 knockdown regardless of the amount of p45/NF-E2 in K562 cells. These results indicate that histone acetylation is dependent on GATA-1 binding, but the binding of GATA-1 is not sufficient for the γ-globin transcription, HS formation and chromatin loop formation and NF-E2 is required. This idea is supported by the distinctive binding pattern of CBP and Brg1 in the β-globin locus. Furthermore GATA-1-dependent loop formation between HS5 and 3′HS1 suggests correlation between histone modifications and chromatin looping.
- URI
- https://academic.oup.com/nar/article/39/16/6944/2411224
- ISSN
- 0305-1048
- DOI
- 10.1093/nar/gkr253
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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