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dc.contributor.author이근용-
dc.date.accessioned2018-02-22T13:19:05Z-
dc.date.available2018-02-22T13:19:05Z-
dc.date.issued2012-06-
dc.identifier.citationBIOCONJUGATE CHEMISTRY, 23, 6, 1174-1180en_US
dc.identifier.issn1043-1802-
dc.identifier.urihttps://pubs.acs.org/doi/abs/10.1021/bc2006219-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/40176-
dc.description.abstractThe intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNA and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies specifically targeting T cells are lacking. We hypothesized that chitosan nanoparticles modified with T cell-specific antibodies would be useful for delivering siRNA to T cells. CD7-specific single-chain antibody (scFvCD7) was chemically conjugated to chitosan by carbodiimide chemistry, and nanoparticles were prepared by a complex coacervation method in the presence of siRNA. The mean diameter and zeta potential of the scFvCD7-chitosan/siRNA nanoparticles were approximately 320 nm and +17 mV, respectively, and were not significantly influenced by the coupling of antibody to chitosan. The cellular association of antibody-conjugated nanoparticles to CD4+ T cell lines as well as gene silencing efficiency in the cells was significantly improved compared to nonmodified chitosan nanoparticles. This approach to introducing T cell-specific antibody to chitosan nanoparticles may find useful applications for the treatment of various infectious diseases.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20110017959).en_US
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USAen_US
dc.subjectGENE DELIVERYen_US
dc.subjectRNA INTERFERENCEen_US
dc.subjectDNA NANOPARTICLESen_US
dc.subjectHIV-1 INFECTIONen_US
dc.subjectSYSTEMSen_US
dc.subjectAPOPTOSISen_US
dc.subjectTHERAPYen_US
dc.subjectCARRIERen_US
dc.titleT Cell-Specific siRNA Delivery Using Antibody-Conjugated Chitosan Nanoparticlesen_US
dc.typeArticleen_US
dc.relation.no6-
dc.relation.volume23-
dc.identifier.doi10.1021/bc2006219-
dc.relation.page1174-1180-
dc.relation.journalBIOCONJUGATE CHEMISTRY-
dc.contributor.googleauthorLee, Jangwook-
dc.contributor.googleauthorYun, Kyoung-Soo-
dc.contributor.googleauthorChoi, Chang Seon-
dc.contributor.googleauthorShin, Seung-Hwa-
dc.contributor.googleauthorBan, Hong-Seok-
dc.contributor.googleauthorRhim, Taiyoun-
dc.contributor.googleauthorLee, Sang Kyung-
dc.contributor.googleauthorLee, Kuen Yong-
dc.relation.code2012201260-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidleeky-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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