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dc.contributor.author최제민-
dc.date.accessioned2018-02-22T08:37:13Z-
dc.date.available2018-02-22T08:37:13Z-
dc.date.issued2012-10-
dc.identifier.citationPLOS ONE, Vol.7, No.10 [2012], p1-12en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0047971-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/40039-
dc.description.abstractThe activity of the serine protease in the German cockroach allergen is important to the development of allergic disease. The protease-activated receptor (PAR)-2, which is expressed in numerous cell types in lung tissue, is known to mediate the cellular events caused by inhaled serine protease. Alveolar macrophages express PAR-2 and produce considerable amounts of tumor necrosis factor (TNF)-alpha. We determined whether the serine protease in German cockroach extract (GCE) enhances TNF-alpha production by alveolar macrophages through the PAR-2 pathway and whether the TNF-alpha production affects GCE-induced pulmonary inflammation. Effects of GCE on alveolar macrophages and TNF-alpha production were evaluated using in vitro MH-S and RAW264.6 cells and in vivo GCE-induced asthma models of BALB/c mice. GCE contained a large amount of serine protease. In the MH-S and RAW264.7 cells, GCE activated PAR-2 and thereby produced TNF-alpha. In the GCE-induced asthma model, intranasal administration of GCE increased airway hyperresponsiveness (AHR), inflammatory cell infiltration, productions of serum immunoglobulin E, interleukin (IL)-5, IL-13 and TNF-alpha production in alveolar macrophages. Blockade of serine proteases prevented the development of GCE induced allergic pathologies. TNF-alpha blockade also prevented the development of such asthma-like lesions. Depletion of alveolar macrophages reduced AHR and intracellular TNF-alpha level in pulmonary cell populations in the GCE-induced asthma model. These results suggest that serine protease from GCE affects asthma through an alveolar macrophage and TNF-alpha dependent manner, reflecting the close relation of innate and adaptive immune response in allergic asthma model.en_US
dc.description.sponsorshipThis study was supported by the grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A092076). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAen_US
dc.subjectPROTEASE-ACTIVATED RECEPTOR-2en_US
dc.subjectAIRWAY EPITHELIAL-CELLSen_US
dc.subjectINNATE IMMUNE-RESPONSEAlveolar Macrophages Play a Key Role inen_US
dc.subjectGERMAN-COCKROACHen_US
dc.subjectDUST-MITEen_US
dc.subjectIL-8 EXPRESSIONen_US
dc.subjectLUNG-DISEASEen_US
dc.subjectASTHMAen_US
dc.subjectEXPOSUREen_US
dc.subjectSENSITIZATIONen_US
dc.titleAlveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-alpha Pathwayen_US
dc.typeArticleen_US
dc.relation.no10-
dc.relation.volume7-
dc.identifier.doi10.1371/journal.pone.0047971-
dc.relation.page1-12-
dc.relation.journalPLOS ONE-
dc.contributor.googleauthorSohn, Jung-Ho-
dc.contributor.googleauthorLee, Jae-Hyun-
dc.contributor.googleauthorHong, Chein-Soo-
dc.contributor.googleauthorPark, Jung-Won-
dc.contributor.googleauthorKim, Joo-Young-
dc.contributor.googleauthorChoi, Je-Min-
dc.contributor.googleauthorLee, Joo-Shil-
dc.relation.code2012219766-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidjeminchoi-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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