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Wash-free magnetic immunoassay of the PSA cancer marker using SERS and droplet microfluidics

Title
Wash-free magnetic immunoassay of the PSA cancer marker using SERS and droplet microfluidics
Author
주재범
Keywords
ENHANCED RAMAN-SCATTERING; PROSTATE-SPECIFIC ANTIGEN; MOLECULARLY IMPRINTED POLYMER; SINGLE-MOLECULE; TRACE ANALYSIS; FOLLOW-UP; SURFACE; SPECTROSCOPY; BIOMARKERS; CELLS
Issue Date
2016-03
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v. 16, NO 6, Page. 1022-1029
Abstract
We report a novel wash-free magnetic immunoassay technique for prostate-specific antigen (PSA) that uses a surface-enhanced Raman scattering (SERS)-based microdroplet sensor. The magnetic bar embedded in a droplet-based microfluidic system segregates the free and bound SERS tags by splitting the droplets into two smaller parts. The presence of PSA targets leads more SERS tags to immunocomplex in one droplet so that fewer SERS tags remain in another supernatant solution droplet. Thus, SERS signal measurement enables the quantitative evaluation of PSA markers. This approach can provide a rapid and sensitive assay that is applicable for PSA cancer markers in serum without any washing. Specifically, SERS signals were measured at 174 droplets per minute and averaged for quantitative evaluation of PSA. The limit of detection (LOD) determined by our SERS-based microdroplet sensor was estimated to be below 0.1 ng mL(-1), which is significantly below the clinical cut-off value for the diagnosis of prostate cancer. In addition, because the entire assay can be carried out automatically, only a minimal amount of sample is needed. Accordingly, the approach is expected to be useful as a potential clinical tool for the early diagnosis of prostate cancer.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2016/LC/C5LC01249J#!divAbstracthttp://hdl.handle.net/20.500.11754/39623
ISSN
1473-0197; 1473-0189
DOI
10.1039/c5lc01249j
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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