Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus
- Title
- Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus
- Author
- 신경훈
- Keywords
- Copepod; Tigriopus japonicus; AhR; ARNT; Polychlorinated biphenyl; Benzo[a]pyrene; IMMUNOPOSITIVE PROTEINS; CYTOCHROME-P450 GENES; CYP GENES; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; ELEGANS; IDENTIFICATION; CYTOSKELETAL; XENOBIOTICS; MECHANISMS; DIVERSITY
- Issue Date
- 2015-02
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Citation
- CHEMOSPHERE, v. 120, Page. 398-406
- Abstract
- The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (ARNT) genes from the copepod Tigriopus japonicus (Tj) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 mu g L-1), benzo[a]pyrene (B[a]P) (control, 5, 10, 50, 100 mu g L-1), and tributyltin (TBT) (control, 1, 5, 10, 20 mu g L-1) 24 h after exposure. A time-course experiment (0, 3, 6, 12, 24 h) was performed to analyze mRNA expression patterns after exposure to PCB, B[a]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[a]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0045653514010042?via%3Dihubhttp://hdl.handle.net/20.500.11754/39381
- ISSN
- 0045-6535; 1879-1298
- DOI
- 10.1016/j.chemosphere.2014.07.099
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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