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Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus

Title
Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus
Author
신경훈
Keywords
Copepod; Tigriopus japonicus; AhR; ARNT; Polychlorinated biphenyl; Benzo[a]pyrene; IMMUNOPOSITIVE PROTEINS; CYTOCHROME-P450 GENES; CYP GENES; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; ELEGANS; IDENTIFICATION; CYTOSKELETAL; XENOBIOTICS; MECHANISMS; DIVERSITY
Issue Date
2015-02
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
CHEMOSPHERE, v. 120, Page. 398-406
Abstract
The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (ARNT) genes from the copepod Tigriopus japonicus (Tj) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 mu g L-1), benzo[a]pyrene (B[a]P) (control, 5, 10, 50, 100 mu g L-1), and tributyltin (TBT) (control, 1, 5, 10, 20 mu g L-1) 24 h after exposure. A time-course experiment (0, 3, 6, 12, 24 h) was performed to analyze mRNA expression patterns after exposure to PCB, B[a]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[a]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0045653514010042?via%3Dihubhttp://hdl.handle.net/20.500.11754/39381
ISSN
0045-6535; 1879-1298
DOI
10.1016/j.chemosphere.2014.07.099
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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