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In situ facile-forming PEG cross-linked albumin hydrogels loaded with an apoptotic TRAIL protein

Title
In situ facile-forming PEG cross-linked albumin hydrogels loaded with an apoptotic TRAIL protein
Author
최한곤
Keywords
Hydrogel; Polyethylene glycol; Albumin; In situ formation; TNF-related apoptosis-inducing ligand; Pancreatic cancer; HUMAN SERUM-ALBUMIN; LIGAND TRAIL; CRYSTAL-STRUCTURE; MOUSE SKIN; DELIVERY; RELEASE; DRUG; PEGYLATION; CHITOSAN; CARRIER
Issue Date
2015-09
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF CONTROLLED RELEASE, V. 214, Page. 30-39
Abstract
The key to making a practicable hydrogel for pharmaceutical or medical purposes is to endow it with relevant properties, i.e., facile fabrication, gelation time-controllability, and in situ injectability given a firm basis for safety/biocompatibility. Here, the authors describe an in situ gelling, injectable, albumin-cross-linked polyethylene glycol (PEG) hydrogel that was produced using a thiol-maleimide reaction. This hydrogel consists of two biocompatible components, namely, thiolated human serum albumin and 4-arm PEG(20k)-maleimide, and can be easily fabricated and gelled in situ within 60 s by simply mixing its two components. In addition, the gelation time of this system is controllable in the range 15 s to 5 min. This hydrogel hardly interacted with an apoptotic TRAIL protein, ensuring suitable release profiles that maximize therapeutic efficacy. Specifically, tumors (volume: 278.8 mm(3)) in Mia Paca-2 cell-xenografted BALB/c nu/nu mice treated with the TRAIL-loaded HSA-PEG hydrogel were markedly smaller than mice treated with the hydrogel prepared via an amine-N-hydroxysuccinimide reaction or non-treated mice (1275.5 mm(3) and 1816.5 mm(3), respectively). We believe that this hydrogel would be a new prototype of locally injectable sustained-release type anti-cancer agents, and furthermore offers practical convenience for a doctor and universal applicability for a variety of therapeutic proteins. (C) 2015 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0168365915300262http://hdl.handle.net/20.500.11754/39286
ISSN
0168-3659; 1873-4995
DOI
10.1016/j.jconrel.2015.07.012
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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