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HPLC Determination of Esculin and Esculetin in Rat Plasma for Pharmacokinetic Studies

Title
HPLC Determination of Esculin and Esculetin in Rat Plasma for Pharmacokinetic Studies
Author
유혜현
Keywords
OXIDATIVE DNA-DAMAGE; COUMARIN COMPOUNDS; AESCULIN; CHROMATOGRAPHY; FRAXINUS
Issue Date
2015-09
Publisher
OXFORD UNIV PRESS INC
Citation
JOURNAL OF CHROMATOGRAPHIC SCIENCE, v. 53, No. 8, Page. 1322-1327
Abstract
An optimized, sensitive and validated reversed-phase high-performance liquid chromatography (RP-HPLC) method with UV detection is described for simultaneous determination of esculin and its aglycone, esculetin, in rat plasma. After addition of internal standard (chrysin), plasma samples were pretreated by solid-phase extraction and introduced into the HPLC system. Analytes were separated on a RP C18 column with a mobile phase of 0.075% acetic acid in water (solvent A) and 90% acetonitrile in solvent A (solvent B) using gradient elution at a flow rate of 1.0 mL/min. The wavelength for UV detection was set at 338 nm. Calibration curves for esculin and esculetin were constructed over a range of 10-1,000 ng/mL. The developed method was found to be specific, precise and accurate. The method was successfully applied to study the pharmacokinetics of esculin and esculetin in rats. After oral administration of 120 mg/kg, the mean C-max values were 340.3 and 316.5 ng/mL and the AUC(last) values were 377.3 and 1276.5 h ng/mL for esculin and esculetin, respectively. The bioavailability of esculin was calculated to be 0.62%.
URI
https://academic.oup.com/chromsci/article/53/8/1322/312685http://hdl.handle.net/20.500.11754/39279
ISSN
0021-9665; 1945-239X
DOI
10.1093/chromsci/bmv014
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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