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Glycogen synthase kinase 3-beta phosphorylates novel S/T-P-S/T domains in Notch1 intracellular domain and induces its nuclear localization

Title
Glycogen synthase kinase 3-beta phosphorylates novel S/T-P-S/T domains in Notch1 intracellular domain and induces its nuclear localization
Author
신인철
Keywords
GSK-3 beta; NLS; Notch; S/T-P-S/T domain; NUCLEOCYTOPLASMIC TRANSPORT; KINASE-3-BETA; SIGNAL; HES1; PATHWAY; ACCESS; BRCA2
Issue Date
2012-06
Publisher
Elsevier
Citation
Biochemical and Biophysical Research Communications,Vol423,No2[2012],p282~288
Abstract
We identified two S/T-P-S/T domains (2122-2124, 2126-2128) inducing Notch intracellular domain (NICD) nuclear localization. The GFP-NICD (1963-2145) fusion protein deletion mutant without classical NLS was localized in the nucleus like the full length GFP-NICD. However, quadruple substitution mutant (T2122A T2124A S2126A T2128A) showed increased cytoplasmic localization. GSK-3 beta enhanced nuclear localization and transcriptional activity of WT NICD but not of quadruple substitution mutant. In vitro kinase assays revealed that GSK-3 beta phosphorylated S and T residues in NICD S/T-p-S/T domains. These results suggest that the novel SIT-P-SIT domain, phosphorylated by GSK-3 beta is also involved in the nuclear localization of NICD as well as classical NLS. (C) 2012 Elsevier Inc. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0006291X12010017?via%3Dihubhttp://hdl.handle.net/20.500.11754/39272
ISSN
0006-291X
DOI
10.1016/j.bbrc.2012.05.111
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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