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dc.contributor.author황승용-
dc.date.accessioned2018-02-19T05:47:46Z-
dc.date.available2018-02-19T05:47:46Z-
dc.date.issued2011-10-
dc.identifier.citationIn Sensors & Actuators: B. Chemical 2011, 157(2),735-741en_US
dc.identifier.issn0925-4005-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0925400511004175?via%3Dihub-
dc.description.abstractA novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 mu L/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLANDen_US
dc.subjectDNA ligationen_US
dc.subjectMicromixeren_US
dc.subjectMicropillaren_US
dc.subjectMicronozzleen_US
dc.subjectSerpentine microreactoren_US
dc.subjectPDMS-glass microbiochipen_US
dc.subjectSERPENTINE MICROCHANNELen_US
dc.subjectPOINT MUTATIONSen_US
dc.subjectMIXERen_US
dc.subjectMICROPUMPen_US
dc.subjectSYSTEMSen_US
dc.subjectPCRen_US
dc.titleDNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactoren_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume157-
dc.identifier.doi10.1016/j.snb.2011.05.016-
dc.relation.page735-741-
dc.relation.journalSENSORS AND ACTUATORS B-CHEMICAL-
dc.contributor.googleauthorKo, Yong-Jun-
dc.contributor.googleauthorMaeng, Joon-Ho-
dc.contributor.googleauthorAhn, Yoomin-
dc.contributor.googleauthorHwang, Seung Yong-
dc.relation.code2011208645-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidsyhwang-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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