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DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor

Title
DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor
Author
황승용
Keywords
DNA ligation; Micromixer; Micropillar; Micronozzle; Serpentine microreactor; PDMS-glass microbiochip; SERPENTINE MICROCHANNEL; POINT MUTATIONS; MIXER; MICROPUMP; SYSTEMS; PCR
Issue Date
2011-10
Publisher
ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Citation
In Sensors & Actuators: B. Chemical 2011, 157(2),735-741
Abstract
A novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 mu L/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. (C) 2011 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0925400511004175?via%3Dihub
ISSN
0925-4005
DOI
10.1016/j.snb.2011.05.016
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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