64 0

Sulfur amino acid metabolism in Zucker diabetic fatty rats

Title
Sulfur amino acid metabolism in Zucker diabetic fatty rats
Author
김영미
Keywords
ZDF rat; Sulfur amino acids; Type 2 diabetes; PERFORMANCE LIQUID-CHROMATOGRAPHY; PLASMA HOMOCYSTEINE ELEVATION; METHIONINE LOADING TEST; ADENOSYL-L-METHIONINE; HEPATIC-METABOLISM; CYSTEINE DIOXYGENASE; METHYL-GROUP; BETAINE; LIVER; MICE
Issue Date
2015-08
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
BIOCHEMICAL PHARMACOLOGY, v. 96, No. 3, Page. 256-266
Abstract
The present study was aimed to investigate the metabolomics of sulfur amino acids in Zucker diabetic fatty (ZDF) rats, an obese type 2 diabetic animal model. Plasma levels of total cysteine, homocysteine and methionine, but not glutathione (GSH) were markedly decreased in ZDF rats. Hepatic methionine, homocysteine, cysteine, betaine, taurine, spermidine and spermine were also decreased. There are no significant difference in hepatic S-adenosylmethionine, S-adenosylhomocysteine, GSH, GSH disulfide, hypotaurine and putrescine between control and ZDF rats. Hepatic SAH hydrolase, betaine-homocysteine methyltransferase and methylene tetrahydrofolate reductase were up-regulated while activities of gamma-glutamylcysteine ligase and methionine synthase were decreased. The area under the curve (AUC) of methionine and methionine-d(4) was not significantly different in control and ZDF rats treated with a mixture of methionine (60 mg/kg) and methionine-d(4) (20 mg/kg). Moreover, the AUC of the increase in plasma total homocysteine was comparable between two groups, although the homocysteine concentration curve was shifted leftward in ZDF rats, suggesting that the plasma total homocysteine after the methionine loading was rapidly increased and normalized in ZDF rats. These results show that the AUC of plasma homocysteine is not responsive to the up-regulation of hepatic BHMT in ZDF rats. The present study suggests that the decrease in hepatic methionine may be responsible for the decreases in its metabolites, such as homocysteine, cysteine, and taurine in liver and consequently decreased plasma homocysteine levels. (C) 2015 Elsevier Inc. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S000629521500297Xhttp://hdl.handle.net/20.500.11754/38035
ISSN
0006-2952; 1873-2968
DOI
10.1016/j.bcp.2015.05.014
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE