Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 계명찬 | - |
dc.date.accessioned | 2018-02-19T00:27:09Z | - |
dc.date.available | 2018-02-19T00:27:09Z | - |
dc.date.issued | 2012-06 | - |
dc.identifier.citation | Journal of Biological Chemistry,VOL. 287, NO. 23, pp. 19516 ?19527 | en_US |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://www.jbc.org/content/287/23/19516.full | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/37974 | - |
dc.description.abstract | The prognosis of breast cancer patients is related to the degree of metastasis. However, the mechanisms by which epithelial tumor cells escape from the primary tumor and colonize at a distant site are not entirely understood. Here, we analyzed expression levels of pituitary tumor-transforming gene-1 (PTTG1), a relatively uncharacterized oncoprotein, in patient-derived breast cancer tissues with corresponding normal breast tissues. We found that PTTG1 is highly expressed in breast cancer patients, compared with normal tissues. Also, PTTG1 expression levels were correlated with the degree of malignancy in breast cancer cell lines; the more migratory and invasive cancer cell lines MDA-MB-231 and BT549 displayed the higher expression levels of PTTG1 than the less migratory and invasive MCP7 and SK-BR3 and normal MCF10A cell lines. By modulating PTTG1 expression levels, we found that PTTG1 enhances the migratory and invasive properties of breast cancer cells by inducing epithelial to mesenchymal transition, as evidenced by altered morphology and epithelial/mesenchymal cell marker expression patterns and up-regulation of the transcription factor Snail. Notably, down-regulation of PTTG1 also suppressed cancer stem cell population in BT549 cells by decreasing self-renewing ability and tumorigenic capacity, accompanying decreasing CD44(high) CD24(low) cells and Sox2 expression. Up-regulation of PTTG1 had the opposite effects, increasing sphere-forming ability and Sox2 expression. Importantly, PTTG1-mediated malignant tumor properties were due, at least in part, to activation of AKT, known to be a key regulator of both EMT and stemness in cancer cells. Collectively, these results suggest that PTTG1 may represent a new therapeutic target for malignant breast cancer. | en_US |
dc.description.sponsorship | This work was supported by National Nuclear Technology Program Grant 2008-2003935 and Converging Research Center Program Grant 2011K000877 funded by the Ministry of Education, Science, and Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA | en_US |
dc.subject | TRANSFORMING GENE PTTG | en_US |
dc.subject | TRANSCRIPTION FACTOR SNAIL | en_US |
dc.subject | IN-VITRO PROPAGATION | en_US |
dc.subject | PITUITARY-ADENOMAS | en_US |
dc.subject | INVASIVE FRONT | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | IDENTIFICATION | en_US |
dc.subject | MAINTENANCE | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | SURVIVAL | en_US |
dc.title | PTTG1 Oncogene Promotes Tumor Malignancy via Epithelial to Mesenchymal Transition and Expansion of Cancer Stem Cell Population | en_US |
dc.type | Article | en_US |
dc.relation.no | 23 | - |
dc.relation.volume | 287 | - |
dc.relation.page | 19516-19527 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.contributor.googleauthor | Yoon, Chang-Hwan | - |
dc.contributor.googleauthor | Kim, Min-Jung | - |
dc.contributor.googleauthor | Lee, Hyejin | - |
dc.contributor.googleauthor | Kim, Rae-Kwon | - |
dc.contributor.googleauthor | Lim, Eun-Jung | - |
dc.contributor.googleauthor | Yoo, Ki-Chun | - |
dc.contributor.googleauthor | Lee, Ga-Haeng | - |
dc.contributor.googleauthor | Cui, Yan-Hong | - |
dc.contributor.googleauthor | Oh, Yeong Seok | - |
dc.contributor.googleauthor | Gye, Myung Chan | - |
dc.relation.code | 2012204730 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | mcgye | - |
dc.identifier.orcid | http://orcid.org/0000-0001-5682-3709 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.